Coding

Part:BBa_K648012:Design

Designed by: Jim Rose   Group: iGEM11_Penn_State   (2011-07-04)


LacZa wtih Standard 25 Prefix/Suffix


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part contains the AgeI and NgoMIV sites as part of it's prefix/suffix that allows it to be used in standard 25 assembly.

It was synthesized through PCR oligo synthesis methods using the following primers:

Forward TATTGAATTCGCGGCCGCTTCTAGATGGCCGGCACCATGATTACGGATTCAC (57.74 oC)

Reverse ATAACTGCAGCGGCCGCTACTAGTATTAACCGGTTCACTCCAGCCAGC (55.05 oC)



Source

This part was amplified out of registry stock of LacZa in order to include the standard 25 prefix/suffix

References