![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K642005:Design
Designed by: Wilson Lam, Matthew Orton Group: iGEM11_uOttawa (2011-09-23)
LacI repressor tagged with yBFP and a VP16 activation domain
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1084
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Both LacI and yeast codon optimized BFP were designed without a stop codon to enable the construction of a fusion protein with BFP and the rtTA activation domain.
Source
The LacI repressor was PCR amplified from a plasmid source of a biobrick (BBa_K319007) that was submitted last year by the our team. Yeast codon optimized BFP was synthesized by the uOttawa team using Mr. Gene DNA synthesis. The VP16 activation domain was PCR amplified from a plasmid used by our lab called pDVA38.