Coding

Part:BBa_K642002:Design

Designed by: Wilson Lam, Matthew Orton   Group: iGEM11_uOttawa   (2011-09-22)

cI repressor tagged with yBFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 711
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

cI lambda was PCR amplified without a stop codon to enable the construction of a fusion protein with yeast codon optimized BFP. BFP was also designed without a stop codon for the purposes of integrating into the Ade2 locus of S. cerevisiae. This Ade2 locus contains a terminator region flanking BFP downstream.

Source

The cI repressor was PCR amplified from a previously submitted part (BBa_K105004) made by iGEM08_ESBS-Strasbourg. Yeast codon optimized BFP was synthesized by the uOttawa team using Mr. Gene DNA synthesis.

References