Regulatory

Part:BBa_K641027:Design

Designed by: Nikit Patel   Group: iGEM11_Berkeley   (2011-09-26)


P(gadB) - Stress Promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 150
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 150
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 150
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Different stress promoters respond to different intra- and extracellular stress. Therefore it is key to test a variety of stress promoters and see what promoter gives the best stress response. We have put up many stress promoters for others to use and try.


Source

MG1655 Genomic DNA

References