Reporter

Part:BBa_K640007:Design

Designed by: Emily Hicks   Group: iGEM11_Calgary   (2011-10-28)

Codon optimized Luciferase for chlamydomonas with Hsp70A promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 252
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 655
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1213


Design Notes

Both genes for the subparts were obtained from the chlamydomonas database. Prefix and suffix were added through PCR.


Source

http://www.chlamy.org/chlamydb.html

References

Fuhrmann et al. (2004) 

  Plant Mol. Biol. 55, 869-881

Heitzer and Zschoernig (2007) 

  Biotechniques 43, 324