Coding

Part:BBa_K638404:Design

Designed by: Joseph Harvey   Group: iGEM11_Cambridge   (2011-09-21)


polyHis-ReflectinA1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 43
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

na


Source

The reflectin A1 coding sequence was back-translated and codon-optimized for E coli. It is synthetic and was kindly given to us by Dr Wendy Crookes-Goodson. The 6-His tag was added by us using synthetic oligonucleotides.

References