RNA

Part:BBa_K635000:Design

Designed by: Arjun Athreya   Group: iGEM11_Virginia   (2011-09-27)


siRNA gene silencer for use in yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The siRNA is intended to be a modular part that can be used in any circuit so long as the gene of interest is preceded by the corresponding random buffer region GGAGTTGTGGGCACCTGCTA and the kozak sequence BBa_J63003. The siRNA therefore was designed to be reverse complementary to the sequence mentioned above.


Source

This part is a novel sequence design. It is complementary to a unique buffer sequence and the kozak sequence (BBa_J63003.)

References