Intermediate

Part:BBa_K634011:Design

Designed by: Kenneth O. Xu   Group: iGEM11_Wisconsin-Madison   (2011-09-23)


sacB + KanR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As a counter-selection marker, sacB is very susceptible to spontaneous mutation.


Source

Cloning from BBa_K322921, BBa_P1003. BBa_P1003 was amplified from PSB4k5.

References