Coding

Part:BBa_K633002:Design

Designed by: Ricardo Camilo Chavez Martinez   Group: iGEM11_Tec-Monterrey   (2011-09-27)


RBS+signal peptide phoA+Cellulase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1908
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 721
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 585
    Illegal AgeI site found at 228
    Illegal AgeI site found at 644
    Illegal AgeI site found at 1473
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since this part is supposed to be attached to part BBa_K633001 in order to geerate part BBa_K633014, we had to begin with both the signal peptide and then the extracelular domain, which is located on the N terminal side of the polypeptide chain.


Source

Source

References