Regulatory

Part:BBa_K632003:Design

Designed by: Lauren Cole   Group: iGEM11_Minnesota   (2011-09-28)


Ho1-PcyA-cph8, regulatory system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2257
    Illegal BglII site found at 3038
    Illegal XhoI site found at 364
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2591
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ho1 and pcyA were put under the same inducible promoter, and cph8 was put under a separate inducible promoter.


Source

Heme oxigenase (ho1) was obtained from a pMOD plasmid library vector. Phycocyanobilin:ferredoxin oxidoreductase (pcyA) was obtained from genomic DNA from Synechocystis sp. PCC 6803. Cph8 from BioBrick: BBa_I15010.

References