Composite

Part:BBa_K628006:Design

Designed by: Charles Thompson   Group: iGEM11_St_Andrews   (2011-09-20)

Protegrin-1 Kill Switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NotI site found at 164
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Protegrin-1 coding region sequenced by Gene Art assembled by standard biobrick assembly. This has been inserted into the standard iGEM 2011 backbone pSB1C3 which has chloramphenicol resistance.


Source

The part has been optimised for e coli and designed with a pBad strong promoter to allow lyses to be controlled by varying the concentration of arabinose. This could be altered in future applications and a more specific promoter could be used to allow regulation of the switch to be tightly linked to conditions in the proposed environment.

References