Composite
ssTorA_CS-
Part:BBa_K627013:Design
Designed by: iGEM11 Potsdam_Bioware Group: iGEM11_Potsdam_Bioware (2011-09-21)
Fusion of ssTorA, cleavage site of 14_3C protease and b-lactamase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 117
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 143
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 796
Design Notes
This biobrick was built by PCR using the following PCR primers:
- p_TorA-f: CTTCTAGATGAACAATAACGATCTCTTTCAGGCATC
- o_bla_igem_r: CTACTAGTATTAACCGGTCCAATGCTTAATCAGTGAGGCAC
Source
TorA signal sequence and the lactamase was amplified via PCR from pJC354, which originates from two iGEM compatible BioBricks: BBa_K208005 and BBa_I757010. The cleavage site was created, based on the information of the Brenda enzymes database, via 2 oligonucleotides ordered from Sigma-Aldrich.
References
ssTorA - https://parts.igem.org/Part:BBa_K208005
blaFL - https://parts.igem.org/Part:BBa_I757010