Plasmid

Part:BBa_K626000:Design

Designed by: Endre Károly Kristóf   Group: iGEM11_Debrecen_Hungary   (2011-09-01)

TRE-Bax-PolyA in PSB1A3 (expression vector)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 778
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Compatible with RFC-10 and RFC-25.


Source

Artificial and human BAX.

References

Oltvai ZN, Milliman CL, Korsmeyer SJ. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell. 1993 Aug 27;74(4):609-19.

Vieira HL, Haouzi D, El Hamel C, Jacotot E, Belzacq AS, Brenner C, Kroemer G. Permeabilization of the mitochondrial inner membrane during apoptosis: impact of the adenine nucleotide translocator. Cell Death Differ. 2000 Dec;7(12):1146-54.

Wolter, K. G.; Hsu, Y., Smith, C. L., Mechushtan, A., Xi, X., and Youle, R. J. (December 1997). "Movement of BAX from Cytosol to Mitochondria during Apoptosis". Journal of Cell Biology 139 (5): 1281–1292.