DNA

Part:BBa_K625004:Design

Designed by: Michael Eichenberger   Group: iGEM11_ETH_Zurich   (2011-09-21)


Minimal pBR322 pMB1 origin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 588


Design Notes

Checked all sequences necessary for plasmid replication and defined minimal sequence.


Source

PCR on plasmid pSEVA 171 provided by Andreas Bosshard.

References