Plasmid

Part:BBa_K624027:Design

Designed by: Shang-Jui Tsai   Group: iGEM11_NYMU-Taipei   (2011-10-04)


pYMB (shuttle vector between Escherichia coli and Magnetospirillum magneticum AMB-1)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal PstI site found at 1173
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1173
    Illegal NotI site found at 7
    Illegal NotI site found at 1166
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal PstI site found at 1173
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal PstI site found at 1173
    Illegal NgoMIV site found at 920
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2504
    Illegal SapI site found at 1421


Design Notes

to fit Biobrick assembly standards


Source

synthesized [ori+Pmsp1+rep], commercially available pUC19

References