Translational_Unit

Part:BBa_K622006:Design

Designed by: Fumitaka Hashiya   Group: iGEM11_Kyoto   (2011-10-02)

ChitinaseA1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1011
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 361
    Illegal NgoMIV site found at 365
    Illegal NgoMIV site found at 1166
    Illegal NgoMIV site found at 1615
    Illegal NgoMIV site found at 1634
    Illegal AgeI site found at 212
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Start codon of this part is GUG.


Source

Genomic DNA of Streptomyces avermitilis

ChiA1 gene was amplified by using direct colony PCR.

References