Part:BBa_K620003:Design

Tet promoter + BisdB

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 545
  Illegal BamHI site found at 933
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 70
  Illegal AgeI site found at 1342
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1152
  

Design Notes

A repeat sequence in the promoter made PCR assembly and Gibson assembly extremely difficult. Ultramers were ordered to prevent mispriming.

Source

Assembled from BBa_R0040, BBa_K123001, BBa_B0014.