Regulatory
Part:BBa_K619891:Design
Designed by: BYU IGEM team Group: iGEM11_BYU_Provo (2011-09-27)
Mutated prfA thermosensor from listeria
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 18
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 18
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 18
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 18
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The prfA-UTR parent contruct BBa-K619889 has a RBS which has been optimized for E. coli. This version has three mutations, T61A, C67T and A70G.
Source
mutated prfA-UTR