Regulatory

Part:BBa_K619890:Design

Designed by: Julianne Grose   Group: iGEM11_BYU_Provo   (2011-09-27)

mutated prfA thermosensor from listeria


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 18
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 18
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 18
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 18
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

see BBa_K619889, the Shine-Delagarno was mutated from GGGGGA to GGAGGA in order to optimize expression in E. coli, otherwise the thermosensor is the original sequence of prfA-UTR from Listeria monocytogenes. This construct has three mutations, A46G, T56C and T72A

Source

This is the prfA-UTR from Listeria monocytogenes.

References