Coding

Part:BBa_K613022

Designed by: Nadine Guenat, Alina Isakova   Group: iGEM11_EPF-Lausanne   (2011-09-20)

TetR E37A P39K mutant

This is a TetR mutant that carries the E37A P39K double mutation. Part of the EPFL2011 muTetR collection.

It has an altered tet operator sequence recognition compared to the wild-type tetR variant. And prefers the tetO-4C5G operator.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Designed sequence (2 mutations in yellow):

MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVAQKTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCA

LLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFD

HQGAEPAFLFGLELIICGLEKQLKCESGS*


[edit]
Categories
Parameters
None