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Coding

Part:BBa_K613015:Design

Designed by: Nadine Guenat, Lilia Salimova   Group: iGEM11_EPF-Lausanne   (2011-09-20)

TetR E37A W43S T141A mutant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mutations were introduced by PCR-induced mutagenesis, based on informations from [http://www.sciencedirect.com/science/article/pii/S0378111907004623 Krueger et al, 2007] and [http://www.sciencedirect.com/science/article/pii/S0022283697915400 Helbl and Hillen, 1998]

Source

The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced the mutations by site-directed mutagenesis.

References