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Part:BBa_K613000:Design

Designed by: Nadine Guenat, Henrike Niederholtmeyer   Group: iGEM11_EPF-Lausanne   (2011-09-20)


Medium-strength Plac promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The biobrick sequence does include the -10 and -35 regions; however it doesn't include the ribosome binding site.


Source

This part is the UV5 Lac promoter. The UV5 mutation is a double mutation concerning the -10 region of the promoter, which makes the consensus the -10 region of E.coli (change of TATGTT to TATAAT). The -35 region is not consensus sequence: it sequence is TTTACA, when the consensus would be TTGAC. which makes it a medium strenght promoter.

References