Composite

Part:BBa_K611032:Design

Designed by: Nicholas Csicsery   Group: iGEM11_UC_Davis   (2011-09-27)


LacI Promoter Variant #2 in Characterization Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1216
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2394
    Illegal BamHI site found at 1156
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


Design Notes

This part does not have a termination sequence at the end. It is recommended that for optimal results, this part be used in a backbone that includes a terminator (such as pSB1C3).


Source

Mutagenic PCR of the LacI repressible promoter, R0010, and standard BioBrick assembly of parts from the registry.

References