Composite
Part:BBa_K611014:Design
Designed by: Nicholas Csicsery Group: iGEM11_UC_Davis (2011-09-18)
cI Repressible Promoter Characterization Construct
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1008
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 948
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 662
Design Notes
There is no terminator at the end, so the part needs to be used in a plasmid that has a terminator after the BioBrick suffix (such as pSB1A3 or pSB1C3). This part contains the PBad promoter without AraC, thus, a strain that already includes AraC is recommended in order to induce PBad.
Source
Built from existing parts in the registry though BioBrick assembly.