Coding

Part:BBa_K609004:Design

Designed by: Ke Liu   Group: iGEM11_HIT-Harbin   (2011-10-01)


A Nisin resistance gene nisI for Streptococcus thermophilus


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
    Illegal suffix found in sequence at 928
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal SpeI site found at 929
    Illegal PstI site found at 943
    Illegal NotI site found at 15
    Illegal NotI site found at 936
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
    Illegal suffix found in sequence at 929
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal XbaI site found at 24
    Illegal SpeI site found at 929
    Illegal PstI site found at 943
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 556


Design Notes

For a reconstruction of food-grade plasmid.


Source

The gene is found in Lactococcus lactis.

References