Coding

Part:BBa_K609002:Design

Designed by: Ke Liu   Group: iGEM11_HIT-Harbin   (2011-10-01)


human collagen type Ⅲ


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
    Illegal suffix found in sequence at 514
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal SpeI site found at 515
    Illegal PstI site found at 529
    Illegal NotI site found at 15
    Illegal NotI site found at 522
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
    Illegal suffix found in sequence at 515
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal XbaI site found at 24
    Illegal SpeI site found at 515
    Illegal PstI site found at 529
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is designed for Streptococcus thermophilus to produce collagen.


Source

The collagen gene comes from the α1 chain of human collagen type Ⅲ (ColⅢ). And the signal peptide called blpC is found in Streptococcus thermophilus CNRZ1066.

References