Tag

Part:BBa_K608408:Design

Designed by: Rüdiger Trojok   Group: iGEM11_Freiburg   (2011-09-14)

GST tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


Design Notes

The GST-tag has the size of 220 amino acids (roughly 26 KDa). It is fused to the N-terminus of the precipitator protein BBa_K608406 and includes a PreScission Protease domain for cleavage of the GST tag during protein purification. [http://www.gelifesciences.com/aptrix/upp01077.nsf/content/Products?OpenDocument&parentid=976040&moduleid=38868&zone=Proteomics]

The GST domain was cloned in front a GFP and the construct then expressed and purified to test the functionality. The used protocol is attached below under "Usage and Biology" .

Source

http://www.bioss.uni-freiburg.de/toolbox/products.php?PL-37

References