Protein_Domain
Part:BBa_K608406:Design
Designed by: Rüdiger Trojok Group: iGEM11_Freiburg (2011-09-14)
Precipitator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 274
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 100
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 732
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Here we investigated an optimal set of non-conserved aminoacids by analysing large sets of similar proteins and databases. You can use this piece of work as a template to design your own protein and give it any function you like, by simply interchanging aminoacids and fusing other domains on the N or C termini. To guarantee proper folding and to shield off the hydrophobic core, a well studied fragment of an LRR protein coming from hagfish was used. This technique was investigated before
Source
The part is constructed from the following protein sequences (PDB: 2z66) N-Terminal hagfish (PDB: 2z62) C-Terminal hagfish (PDB: 3cvr) bacterial ligase