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Tag
Part:BBa_K608404:Design
Designed by: Sophie Cramer Group: iGEM11_Freiburg (2011-09-14)
IPTG-inducible Promoter with plastic binding domain-tagged GFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 870
Design Notes
To make the plastic binding domain observable we coupled it to GFP as a reporter. Due to the high hydropathy of the plastic binding sequences this protein seems to be toxic to the bacteria. For this reason we used an inducible promoter for the expression of this part. The cells can be cultivated until an optical density of 60-80 followed by an addition of IPTG to induce the expression of the protein.
Source
References
"The nature of target-unrelated peptides recovered in the screening of phage-displayed random peptide libraries with antibodies"