Reporter
T7AmbLoop

Part:BBa_K606064:Design

Designed by: Kvin Yauy   Group: iGEM11_Paris_Bettencourt   (2011-10-23)

T7 amber with autoamplification loop


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3598
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3374


Design Notes

To design the T7 amber we had to consider how many amber sites we would put there for it to be the less leaky possible. Our choice is 2 mutations Amber.


Source

The T7 amber polymerase is from the classic T7 polymerase (part BBa_K145001).


References