Regulatory
pHS

Part:BBa_K606040:Design

Designed by: Axel Séguret   Group: iGEM11_Paris_Bettencourt   (2011-09-18)

Promoter Hyperspank B.subtilis & E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part have been synthetize de novo, by two 80bp primers matching on about 20bp, and completed using phusion polymerase, and then cloned in the pSB1C3 standard plasmid.

Source

The sequence of this part vas inpire by the part BBa_K143015 from Cambridge, but without additional and non necessary sequences before and after the LacO. We created this part because the cambridge part was not available on the registry.

References