Coding

Part:BBa_K602003:Design

Designed by: Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh   Group: iGEM11_Osaka   (2011-10-03)


Deinococcus radiodurans RecA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have designed part according to BBF RFC 54, which modifies the prefix and suffix to allow for the use of shorter PCR cloning primers but can be assembled in the usual way (i.e. Standard Assembly, RFC 10).


Source

Obtained by PCR from genomic DNA.

References