Device

Part:BBa_K598020:Design

Designed by: Zhenrun ZHANG   Group: iGEM11_Peking_R   (2011-09-28)


B0015+pBAD+vioAB+B0015+pBAD+TPP Up-regulated Hammerhead Ribozyme 1.20+vioD+B0015+pBAD+vioE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 262
    Illegal NheI site found at 1296
    Illegal NheI site found at 4826
    Illegal NheI site found at 6466
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 202
    Illegal BamHI site found at 4766
    Illegal BamHI site found at 6406
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4081
    Illegal AgeI site found at 4277
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 5915
    Illegal SapI.rc site found at 5990


Design Notes

The Ribosomal Binding Site of vioD is contained in TPP ribozyme 1.20.


Source

The hammerhead ribozyme comes from E. coli, and the TPP aptamer comes from B. subtilis.

References