Device

Part:BBa_K598013:Design

Designed by: Xiaowei YAN, Tong MU   Group: iGEM11_Peking_R   (2011-09-27)


pBAD+theoHH+CdIntron+E0040+B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1433


Design Notes

The ligand response is alter from c-di-GMP to theophylline by merging a theophylline hammerhead ribozyme to the intron.


Source

The intron derives from the genome of Cd

References