Composite

Part:BBa_K597105:Design

Designed by: James Mathew   Group: iGEM11_Cornell   (2011-09-26)

CCaS and CCaR Light Sensor System


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 606
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2696
    Illegal XhoI site found at 2252
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1270
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CCaS and CCaR are separated by a stop box to prevent transcription run-through. Additionally, there is an RBS after the stop box, to insure the transcription of CCaR. Finally, CCaR is also flanked by a stop box on its C-terminus.

Sources

Sequences provided by Jeff Tabor (Rice University, Department of BioEngineering)1

DNA synthesized through GeneArt, a subsidiary of Invitrogen Life Technologies

References

1. Tabor, J (2011) "Multichromatic control of gene expression in escherichia coli." Journal of Molecular Biology Vol. 405, 2, 2011, p. 315