Composite

Part:BBa_K594014:Design

Designed by: Yu Xiaoyan   Group: iGEM11_OUC-China   (2011-10-01)


A device that can accepts the 3--OH-C14:1-HSL and then produces 3-O-C6-HSL and GFP reporter.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1900
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 608
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2577


Design Notes

all the parts are from the iGEM, including K204032, C0261, E0240


Source

When ligate the RBS with others, 3A assembly is not as efficient as standard assembly.


References