Composite

Part:BBa_K592018:Design

Designed by: Lei Sun   Group: iGEM11_Uppsala-Sweden   (2011-09-21)


B0034-cph8


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 382
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since Cph8 was considerably longer than the RBS added, sequencing was necessary to confirm the construct.


Source

The cph8 was taken from Tabor's pJT122 plasmid.

References