Coding

Part:BBa_K592002:Design

Designed by: Erik Lundin   Group: iGEM11_Uppsala-Sweden   (2011-09-09)

ccaR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 402
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The source DNA (pJT122) contained illegal restriction site (EcoRI) that had to be removed. Point mutagenesis of A4C and A6C were performed.


Source

Synechocystic sp. PCC6803, Jeffrey Tabor's pJT122 plasmid

References

Tabor, J. J., Levskaya, A., and Voigt, C. A. (2011). Multichromatic control of gene expression in Escherichia coli. J. Mol. Biol. 405, 2, 315–324.