Coding
cph8
Part:BBa_K592000:Design
Designed by: Lei Sun Group: iGEM11_Uppsala-Sweden (2011-09-18)
cph8 (Cph1/EnvZ fusion)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 364
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The source DNA (pJT122) contained illegal restriction site (PstI) that had to be removed.
Source
Jeffrey Tabor's pJT122 plasmid
References
Tabor, J. J., Levskaya, A., and Voigt, C. A. (2011). Multichromatic control of gene expression in Escherichia coli. J. Mol. Biol. 405, 2, 315–324.