Coding
KumaMax

Part:BBa_K590087:Design

Designed by: Sydney Gordon, Daniel Hadidi, Elizabeth Stanley, Sarah Wolf, Angus Toland, Sean Wu   Group: iGEM11_Washington   (2011-09-22)

KumaMax: Kumamolisin-As_N291D, G319S, D358G, D368H


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1696
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 447
    Illegal NgoMIV site found at 720
    Illegal NgoMIV site found at 1248
    Illegal NgoMIV site found at 1494
    Illegal AgeI site found at 772
    Illegal AgeI site found at 1348
    Illegal AgeI site found at 1588
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Point mutations at residues 291, 319, 358, and 368 from Asparagine to Aspartate, Glycine to Serine, Aspartate to Glycine, and Aspartate to Histidine, respectively.

Source

Engineered but the original protein is form Alicyclobacillus sendaiensis

References