Coding
mamPA

Part:BBa_K590006:Design

Designed by: Michael Brasino, Alicia Wong, Rashmi Ravichandran   Group: iGEM11_Washington   (2011-09-15)

mamPA, from Magnetospirillum magneticum


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 399
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 399
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 399
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 399
    Illegal NgoMIV site found at 269
    Illegal NgoMIV site found at 456
    Illegal NgoMIV site found at 1493
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

sequence confirmed


Source

Extracted from host genome Magnetospirillum magneticum strain AMB-1

References

D. Murat Quinlan, H. Vali, and A. Komeili. A Comprehensive genetic dissection of the magnetosome gene island reveals the step-wise assembly of a prokaryotic organelle. PNAS. 2010,  vol. 107, 12.