Coding
mamE

Part:BBa_K590001

Designed by: Michael Brasino, Alicia Wong, Rashmi Ravichandran   Group: iGEM11_Washington   (2011-09-15)

mamE, from Magnetospirillum magneticum

This part was extracted from the genome of Magnetospirillum magneticum strain AMB-1 by [http://2011.igem.org/Team:Washington UW iGEM Team 2011]

Usage and Biology

mamE is 2172bp in length and its gene number is AMB-0963. It was reported as membrane-bound serine protease required for magnetite formation; it might also associate with the localization of other magnetosome proteins.

The image below is the gel of mamE with expected length of 2172bp.

1kb ladder (left), mamE (right)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 884
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 884
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2084
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 884
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 884
    Illegal AgeI site found at 1558
    Illegal AgeI site found at 1973
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/amamE, from Magnetospirillum magneticum