Coding

Part:BBa_K582001:Design

Designed by: Mitesh Agrawal   Group: iGEM11_GeorgiaTech   (2011-09-28)


Cas9 genome from the Streptococcus Thermophilus LMD-9 strain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3202


Design Notes

We had to mutate the restriction sites present in the cas9 sequence which are present in the biobrick.


Source

http://www.ncbi.nlm.nih.gov/nuccore/116626972?report=graph&from=1379975&to=1384141

References