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Part:BBa_K578002:Design

Designed by: Kylie Standage-Beier   Group: iGEM11_Arizona_State   (2011-09-27)


T7LRSR (Listeria innocua CLIP11262)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is intended to be the starting point of a constructible CRISPR array, thus no scars can be introduced in between the Leader Sequence and and initial repeat. Also when constructing an array close to a 100% sequence similarity between the spacer and protospacer is required for CRISPR effector function.


Source

This part is based off of the genomic sequence for Listeria Innocua CLIP11262 and was synthesized by Integrate DNA Technologies

References