Reporter

Part:BBa_K577895:Design

Designed by: Kyle Jones   Group: iGEM11_BU_Wellesley_Software   (2011-09-15)


TetR repressed RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1529
    Illegal AgeI site found at 1641
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The whole part is in the backbone of I14033. P0440, the TetR composite part was used and inserted into its respective promoter. The RBS+RFP+Terminator part came from the backbone J61102 and was inserted into the pTet promoter. The pTet+RFP part was then combined as a suffix insertion to the I14033+TetR backbone.


Source

Spring 2011 iGEM Kit Plates

References