Coding
Part:BBa_K576002:Design
Designed by: George Zarubin Group: iGEM11_Waterloo (2011-06-20)
GFP2 TailPart
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 186
Illegal SapI site found at 3
Design Notes
The reading frame fix in the front is unnecessary. The part is in recommended RFC53 D-Tail format, i.e. the overhang is 5' ATG.
Source
BBa_I715020