Part:BBa_K575043:Design

Genomic RhlR/PAI2 Inducible Promoter + RBS (B0030) + GFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 304
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 357
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1194
  

Design Notes

The primers used to extract the promoter had the biobrick restriction sites to ensure we could make it compatible in an assembly such as this.

Source

The promoter is from the Pseudomonas Aeruginosa genome, the other parts are from the registry.