Composite

Part:BBa_K575041:Design

Designed by: Kristin Palarz   Group: iGEM11_Northwestern   (2011-09-22)

Genomic LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1190


Design Notes

The primers used to extract the promoter had the biobrick restriction sites to ensure we could make it compatible in an assembly such as this.


Source

The promoter is from the Pseudomonas Aeruginosa genome, the other parts are from the registry.

References