Composite

Part:BBa_K575039:Design

Designed by: Kristin Palarz   Group: iGEM11_Northwestern   (2011-09-22)

RhlR/PAI2 Inducible Promoter + RBS (B0034) + RFP + Constitutive Promoter + RBS (B0034) + RhlR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 800
    Illegal NheI site found at 823
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1094
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 634
    Illegal AgeI site found at 746
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1569


Design Notes

The constitutive promoter is located downstream of the inducible promoter because the reverse alignment may have resulted in unwanted leaky transcription of GFP.

Source

The pieces of this construct are all existing registry parts.

References