Composite

Part:BBa_K575032:Design

Designed by: Kristin Palarz   Group: iGEM11_Northwestern   (2011-09-22)

RhlR/PAI2 Inducible Promoter + RBS (B0030) + GFP + Constitutive Promoter + RBS (B0030) + RhlR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 817
    Illegal NheI site found at 840
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1114
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1589
    Illegal BsaI.rc site found at 726

Design Notes

The constitutive promoter is located downstream of the inducible promoter because the reverse alignment may have resulted in unwanted leaky transcription of GFP.

Source

The pieces of this construct are all existing registry parts.

References